Cell Biology Atlas 96

CELL BIOLOGY ATLAS OF ELECTRON MICROGRAPHS

Plate No.	Description
1.	Diagrammatic Representation of Hepatocytes - Plasma membrane Domains.
2.	Organization of the Liver - Light Microscopy and TEM
3.	Schematic Diagram of a Hepatocyte.
4.	Rat Hepatocytes (TEM)
5.	Monolayer of Cultured MDCK Cells (Madin-Darby Canine Kidney Cell Line) Examined by Different Techniques (Scanning Electron Microscope, SEM; TEM; Freeze-Fracture)
6.	Junctional Complex (TEM)
7.	Freeze-Fracture Appearance of Tight Junctions and Gap Junctions (TEM of Replicas)
8.	Gap Junction (TEM)
9.	Basal and Lateral Membrane Specializations (TEM)
10.	Basal Infoldings (TEM)
11.	Labeling of Plasma Membrane Components (Immunocytochemistry)
12.	Nucleus and Nucleolus (TEM)
13.	Freeze-Fracture Electron Micrograph of Hepatocyte Showing P (outer membrane) and E (inner membrane) Fracture Faces of the Nuclear Envelope
14.	High Magnification Thin Section Electron Micrograph of a Rat Exocrine Pancreatic Cell (TEM)
15.	Ribosome Structure
16.	Rough and Smooth Endoplasmic Reticulum (TEM)
17.	Smooth Endoplasmic Reticulum (TEM)
18.	Golgi Apparatus and Centriole (TEM)
19.	Immunolabeling of the Endoplasmic Reticulum
20.	Immunolabeling of Golgi Apparatus
21.	Golgi Apparatus (TEM)
22.	Centrioles (TEM)
23.	Mitochondria and Perosixomes (TEM)
24.	Plasma Membrane and Clathrin-Coated Vesicles
25.	Endocytic and Lysosomal Compartments (TEM)
26.	Mouse Macrophages Ingesting Red Blood Cells (SEM)
27.	Microtubules and Microfilaments (TEM)
28.	Actin Cytoskeleton (TEM)
29.	Detection of Actin and Keratin by Immunofluorescence
30.	Cilia (TEM)
31.	Microtubules as seen by Immunofluorescence and TEM
32.	Self-Test Electron Micrograph. Rat Hepatocyte (TEM)
33.	Self-Test Electron Micrograph. MDCK Cell (Freeze-Fracture Replica - TEM)

Some of these electron micrographs are integrated into a computer module (Hippocrates Project) serving as a self test. The Mcintosh computers are available in the Computer Media Center (see also page IN-3).

Editor: Carmen De Lemos-Chiarandini

ATLAS OF IMMUNOFLUROESCENCE MICROGRAPHS

Microscope Slides for Immunofluorescence Laboratory -- Cell Bio Web Page

These slides of cultured cells on coverslips seen in the Filaments and Junctions immunofluorescence laboratory are being made available on the web.

Plate No. Description

1. Fibroblasts stained with mouse monoclonal antibody directed against tubulin.
2. Fibroblasts treated with vinblastine stained with mouse monoclonal antibody directed against tubulin followed by rhodamine-conjugated donkey anti-mouse IgG.
3. Fibroblasts stained with fluorescein (FITC)-conjugated phalloidin (actin stress fibers).
4. Fibroblasts (same as #4) stained with mouse antiserum directed against vinculin (focal adhesion contacts) followed by rhodamine-conjugated donkey anti-mouse IgG
5. MDCK epithelial cells stained with FITC phalloidin. Note circumferential filament bundles.
6. MDCK cells stained with rabbit antiserum directed against occludin (tight junction glycoprotein) followed by rhodamine donkey anti-rabbit IgG.
7. MDCK cells stained with mouse anti-desmoplakin and mouse anti-keratin followed by FITC donkey anti-mouse IgG.
8. MDCK cells stained with mouse anti-E-cadherin followed by rhodamine anti-mouse IgG.
9. Skin stained with a monoclonal antibody against desmoplakin followed by FITC donkey anti-mouse IgG.
10. Skin stained with a monoclonal antibody against keratin 5 followed by FITC donkey anti-mouse IgG
11. Skin stained with a monoclonal antibody against keratin 1 followed by FITC donkey anti-mouse IgG.
12. In panel d, cardiac muscle stained with monoclonal antibody against desmoplakin

Dontated by: Drs. Mickey Rindler and Pam Cowin.

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